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How-To Guide

Peptide Dosage Calculator: BPC-157, TB-500, Semaglutide & More

Scientific Aminos Research TeamOctober 17, 202510 min

Calculate exact peptide doses with our comprehensive guide. Includes dosage charts for BPC-157, TB-500, Semaglutide, and reconstitution math explained simply.

Peptide Dosage Calculator Guide

Calculating accurate peptide doses requires understanding concentrations and syringe measurements. This guide provides formulas, charts, and examples for common peptides.

Research Disclaimer
This article is for educational and research purposes only. The information provided does not constitute medical advice. Consult qualified healthcare professionals before making any health-related decisions.

The Master Formula

Volume to Draw (mL) = Desired Dose (mcg) ÷ Concentration (mcg/mL)

Or in simpler terms:

Syringe Units = (Desired Dose ÷ Peptide Amount) × Water Added × 100

Understanding Insulin Syringes

Standard U-100 insulin syringes:

  • 1mL syringe = 100 units total
  • 0.5mL syringe = 50 units total
  • 0.3mL syringe = 30 units total

Key conversion:

  • 10 units = 0.1mL
  • 1 unit = 0.01mL

Quick Reference Dosage Charts

BPC-157 Dosage Chart

5mg vial reconstituted with 2mL BAC water = 2.5mg/mL (2,500mcg/mL)

Desired DoseVolumeSyringe Units
100mcg0.04mL4 units
200mcg0.08mL8 units
250mcg0.10mL10 units
300mcg0.12mL12 units
500mcg0.20mL20 units

Research dosing notes:

  • Typical research range: 200-500mcg
  • Often administered 1-2x daily in studies
  • Local vs systemic administration may affect protocols

TB-500 Dosage Chart

5mg vial reconstituted with 2mL BAC water = 2.5mg/mL

Desired DoseVolumeSyringe Units
1mg0.40mL40 units
2mg0.80mL80 units
2.5mg1.00mL100 units
5mg2.00mLFull vial

Research dosing notes:

  • Loading phase often uses higher amounts
  • Maintenance typically lower
  • Usually 1-2x weekly in research

Semaglutide Dosage Chart

5mg vial reconstituted with 2.5mL BAC water = 2mg/mL (2,000mcg/mL)

Desired DoseVolumeSyringe Units
0.25mg (250mcg)0.125mL12.5 units
0.5mg (500mcg)0.25mL25 units
1mg0.50mL50 units
1.7mg0.85mL85 units
2.4mg1.20mLNeed 2 draws

Retatrutide Dosage Chart

10mg vial reconstituted with 2mL BAC water = 5mg/mL

Desired DoseVolumeSyringe Units
1mg0.20mL20 units
2mg0.40mL40 units
4mg0.80mL80 units
5mg1.00mL100 units

GHK-Cu Dosage Chart

50mg vial reconstituted with 2mL BAC water = 25mg/mL

Desired DoseVolumeSyringe Units
1mg0.04mL4 units
2mg0.08mL8 units
5mg0.20mL20 units
10mg0.40mL40 units

Wolverine Stack (BPC-157 + TB-500)

Common research combination. Prepare each vial separately:

BPC-157 (5mg in 2mL): 250mcg = 10 units TB-500 (5mg in 2mL): 2.5mg = 100 units (or 2mg = 80 units)

Step-by-Step Calculation Examples

Example 1: BPC-157

Given:

  • 5mg BPC-157 vial
  • Added 2mL bacteriostatic water
  • Want 300mcg dose

Calculate:

  1. Concentration = 5mg ÷ 2mL = 2.5mg/mL = 2,500mcg/mL
  2. Volume = 300mcg ÷ 2,500mcg/mL = 0.12mL
  3. Syringe units = 0.12 × 100 = 12 units

Example 2: Semaglutide

Given:

  • 5mg semaglutide vial
  • Added 2.5mL bacteriostatic water
  • Want 0.5mg dose

Calculate:

  1. Concentration = 5mg ÷ 2.5mL = 2mg/mL
  2. Volume = 0.5mg ÷ 2mg/mL = 0.25mL
  3. Syringe units = 0.25 × 100 = 25 units

Example 3: Custom Calculation

Given:

  • 10mg peptide vial
  • Added 3mL bacteriostatic water
  • Want 750mcg dose

Calculate:

  1. Concentration = 10mg ÷ 3mL = 3.33mg/mL = 3,333mcg/mL
  2. Volume = 750mcg ÷ 3,333mcg/mL = 0.225mL
  3. Syringe units = 0.225 × 100 = 22.5 units (round to 22 or 23)

Choosing Your BAC Water Volume

The amount of bacteriostatic water you add affects ease of measurement:

ApproachProsCons
Less water (1mL)Fewer injections, concentratedHarder to measure small doses
More water (2-3mL)Easy to measure preciselyMore volume per dose

Recommendation: For peptides dosed under 500mcg, use more water for accuracy. For higher doses (mg range), less water is fine.

Reading Your Syringe Correctly

U-100 Insulin Syringe Markings

  • Each small line = 1 unit (0.01mL)
  • Each large line = 5 units (0.05mL)
  • Numbers (10, 20, 30...) = units, not mL

Tips:

  • Hold syringe at eye level
  • Read at the bottom of the meniscus
  • Use appropriate syringe size (don't use 1mL for 5 units)

Common Mistakes

1. Confusing mg and mcg

  • 1mg = 1,000mcg
  • Always double-check your units

2. Wrong Syringe Type

  • Only use U-100 insulin syringes for these calculations
  • U-40 syringes require different math

3. Not Accounting for Dead Space

  • Some syringes retain ~0.05mL in the needle hub
  • For expensive peptides, consider low dead-space syringes

4. Rounding Errors

  • With small doses, rounding matters
  • If between units, go to the nearest whole number

Dosing Frequency Reference

Different peptides have different research protocols:

PeptideTypical Research Frequency
BPC-1571-2x daily
TB-5002x weekly
Semaglutide1x weekly
Retatrutide1x weekly
GHK-CuDaily to 2x daily
CJC-1295/Ipamorelin1-2x daily

Frequently Asked Questions

How many units is 250mcg of BPC-157?

With 5mg in 2mL: 250mcg = 10 units. With 5mg in 1mL: 250mcg = 5 units.

How much BAC water for 10mg retatrutide?

2mL is standard, giving 5mg/mL. This makes 1mg = 20 units, easy to measure.

Can I combine peptides in one syringe?

Research protocols sometimes combine compatible peptides. Draw each separately into the same syringe. Ensure chemical compatibility first.

What if I can't measure exact units?

Round to the nearest whole unit. The difference of 1-2 units is minimal for most research applications.

Summary

  1. Always know your concentration (peptide amount ÷ water added)
  2. Use the formula: Volume = Dose ÷ Concentration
  3. Convert to units: Volume (mL) × 100 = units
  4. Use appropriate syringe for your dose range
  5. Double-check units (mg vs mcg) before calculating
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